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Methods and Techniques
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» Analytical Biochemistry  - Emphasizes methods in the biological and biochemical sciences.
» Assay of Protein Concentration - Different methods of determining protein concentration, as a resource for researchers and students in biochemistry and molecular biology laboratories from University of Nebraska, Lincoln, NE.
» BBHelp - Collection of molecular biology techniques derived from help files of Dr. Harold Zappe at the Department of Medical Microbiology, University of Cape Town, South Africa.
» Basic Principles of Gene Cloning, Sequencing, & Analysis - Description of methods behind gene cloning, genetic engineering, DNA recombination and isolation, including discussion of restriction enzymes and ligase.
» Beginning Laboratory Manual - Teaching manual to support the applied biotechnology programs at the University of Maryland, Baltimore.
» BioProtocol - Internet project initiated in early 1992 as BRNI, bio.com includes PCR, hybridization and microarray protocols.
» Comet Assay Interest Group - Forum for exchange of views on the single cell gel electrophoresis system, capable of detecting single- and double-strand breaks, oxidative DNA base damage, and DNA, protein and drug crosslinking. Includes related links.
» Comparative Genomic Hybridization - CGH is a molecular cytogenetic method of screening a tumor for genetic changes. CGH protocols, images, publications, events and links maintained by the University Hospital Charité, Humboldt-University of Berlin, Germany.
» ECR Browser - Multiple alignment and dynamic evolutionary conservation profile of several vertebrate genomes including human, mouse, rat, chicken, frog, and three fishes, developed at LLNL, Livermore, California.
» Hirsh Lab Gel Documentation System - Description of an agarose gel set-up. Includes instructions for camera operation with a script, with computer side image analysis from the University of Virginia, Charlottesville.
» Home Page of Andy Vierstraete - The use of DNA techniques in phylogenesis. Explanations, protocols and animations from Andy Vierstraete at the University of Ghent, Belgium.
» Journal of Biochemical and Biophysical Methods - Publishes research papers dealing with the development of new methods or the significant modification of existing techniques to solve biological problems.
» MolBio-Spot - Richard Man-kit Yu's site where you can acquire various molecular biology techniques.
» Molbiol.net - Resource for molecular biologists on methods, with feedback for user-directed evolution and links to commercial suppliers from Jonathan D Rees, Oxford, UK.
» Molecular Biology Protocols - Resources such as polymerase chain reaction, dedicated to method development. Part of internet directory maintained by Horizon Scientific Press, Wymondham, England.
» Molecular Cloning Manual - Subscription only source of laboratory techniques in DNA and protein science, to complement the hard copy, giving scientists the benefit of both media from CSHL, NY.
» Molecular Probes, Inc. - Handbook of fluorescence applications, bibliography, data tables and calculators for molecular biology, information on courses and workshops from supplier in Eugene, OR and Leiden, Netherlands.
» Owczarzy, Richard - Online calculations of nucleic acid extinction coefficients, melting temperatures, and links to biophysics databases. Includes thesis, publications and contacts for commercial scientist in Coralville, Iowa.
» Protocols - Microsatellites and others techniques from the Savana River Ecology Laboratory, University of Georgia.
» Recombinant DNA - Preparing probe, cloning into plasmids, complementarity and hybridization, gel electrophoresis, transfer to solid support and blotting variants, blocking, target detection PCR problems from chapter in MIT hypertext handbook.
» Reto's Buffers and Media Book - Alphabetical list for buffer recipes, including constituents for cultures, from a personal page in Switzerland.
» SNP Scoring - Overview of genotyping, detection and quantifying single nucleotide polymorphism, including the application of template directed dye terminator incorporation assay. Offers database and links to other resources from PerkinElmer Life Sciences, Boston, MA.
» SciWeb Protocols and Reagents - Directory of molecular biology protocol links.
» Surgery Research - Collection of DNA microarray technology protocols, primarily for resident students at Stanford.
» Tronolab Website - Collection of protocols, maps and sequences regarding manipulation of lentiviral vectors, siRNA and transgenic animals. Applications in HIV, stem cell and gene therapy research at University of Geneva.
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